Part:BBa_K2155002

CtDock

It is from C.thermocellum CelS (673−741 amino acids), which can automatically assemble with a cohesin module which is from CipA.

Usage and Biology

CtDock is the type I dockerin domain of the Cellulase S (CelS), part of the Clostridium thermocellum cellulosomes. These cellulases contain a dockerin domain that attaches to the cellulosome through cohesin/dockerin binding interactions. The interactions are calcium ion dependent and binding is specific, therefore a type I cohesin will only bind to a type I dockerin. In our project, we fused BFD with CtDocS, and then CtDocS could bind with CtCohesin, which is part of our scaffold protein. Therefore, our core enzyme could form a enzyme complex, and the catalytic efficiency could be greatly improved.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]